I. ISOLATION OF HUMAN, ANIMAL, AND PLANT EVs
- Highly efficient separation of populations of large, medium and small EVs
- EV isolation by ultrafiltration or centrifugation
- Sucrose gradient purification
II. ENRICHMENT OF SPECIFIC EV POPULATIONS
- Immunoselection-based enrichment of specific populations of human, animal, and plant EVs (PDL1+, EGF+, CD44+, EpCAM+, CD117+, CD29+, CD24+, CD66+, CA125+, PSA+, CD105+, CD47+, TET9+ and others)
III. CHARACTERIZATION OF EVs BY ELECTRON MICROSCOPY (TEM)
- Characterization of the size and morphology of extracellular vesicles
- Assessment of EV preparation purity
IV. CHARACTERIZATION OF EVs BY NTA
- Analysis of particle (EVs) size distribution
- Estimation of EV concentration
- Assessment of sample homogeneity and aggregation state
V. ANALYSIS OF PROTEIN MARKERS ON EVs
Single EV flow analysis is a powerful tool for profiling of proteins on large EVs, but often failures to detect pathological markers on small and medium EVs due to its sensitivity is not enough to detect low represented proteins.
- We offer the services of highly sensitive bead-based flow analysis of membrane protein PDL1, EGF, CD44, EpCAM, CD117, CD29, CD24, CD66, CA125, PSA, CD105, CD47 and some others on EVs
- We are also developing custom assays on request.
VI. CONSULTING
We will help you to optimize your experimental protocol:
- To adjust the differential centrifugation protocol to rotor(s) in use to achieve quasi 100% EV isolation yield
- To enrich the EVs population of interest
- To estimate the optimal time for zonal gradient centrifugation
- To optimize the protocol for flow analysis of protein markers on different populations of EVs
- To make the correct choice of tubes, filters for ultrafiltration and other consumables that can essentially decrease the EVs loss during the manipulations