We have developed highly sensitive kits for FACS profiling of CD9, CD81, EpCam, CD44, CD166, CD117, GATA3 and other markers on small, medium and large EVs.
Detection limit for tetraspanins CD9/CD81 on small and medium EVs is 5x10^5. Detection limit for another protein marker (EpCam, CD44, CD166, CD117 and GATA3 and some others) depends on their density on the EV membrane and varies in the range of 3x10^6 to 10^9 EVs.
Our innovative ELISA technology allows reliable detection of 1.5x10^7 CD9/ CD81 small and medium EVs. This sensitivity is 100 times higher than that of conventional ELISA kits applied for EV analysis.
Lateral flow assay for express exosomes quantification and profiling
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